Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Identification of G6PD Aures by an ARMS-PCR assay. a Schematic representation of the primer location and predicted size of PCR products for the G6PD Aures and internal control fragment. b Agarose gel electrophoresis represents band of G6PD Aures (226 bp) and internal control fragments (519 bp). Lane 1 represents the 100 bp DNA marker. Lanes 2–4 represent G6PD Aures-positive samples, and lanes 5–6, G6PD Aures-negative samples

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Control, Agarose Gel Electrophoresis, Marker

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Agarose gel electrophoresis representing 8 G6PD Asian genotypes. Lane 1 represents a 100 bp DNA marker. Lane 2 is a G6PD mutation standard marker. Lanes 3–7 are PCR products of unknown samples, lane 8 is the normal control, and lane 9 is the mutant positive control

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Agarose Gel Electrophoresis, Marker, Mutagenesis, Control, Positive Control

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Prevalence and frequency of G6PD gene mutations in the Lao Theung ethnic group

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques:

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: The histogram shows the correlation between G6PD activity (U/gHb) and G6PD mutations in the Lao Theung population. a G6PD activity (U/gHb) in each G6PD mutation group for males. Enzyme activity below 2.0 U/gHb indicates G6PD deficiency; enzyme activity of 2.0 to 4.67 U/gHb indicates G6PD intermediate. All hemizygous subjects presented G6PD deficiency. b G6PD activity for the female population. Homozygous subjects presented G6PD deficiency, while the heterozygous subjects have intermediate to normal G6PD enzyme activity

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Activity Assay, Mutagenesis

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Prevalence and molecular characteristics of G6PD deficiency in Lao PDR

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Mutagenesis

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Detection of RUNX2 gene expression in cumulus cells in women undergoing controlled ovarian stimulation

doi: 10.1186/1477-7827-10-99

Figure Lengend Snippet: Distribution of RUNX2 gene expression (A) on patient’s population and statistically significant correlations between ovulation induction factors, such as number of follicles, oocytes, fertilized oocytes (B) and hormone serum levels for E2 (C) and LH (D) respectively. RUNX2 gene expression was determined by real-time PCR for 41 patients and categorized into two groups of patients, with expression and without expression. Expression ratios (RUNX2 copies/G6PDH copies) were used for the evaluation of RUNX2 gene expression.

Article Snippet: G6PDH PCR mixtures contained 5 μl G6PDH standard (LightMix Kit human G6PDH, TIB MOLBIOL), 5x master mix (LightCycler 480 Genotyping Master, Roche), 16x G6PDH (LightMix Kit human G6PDH, TIB MOLBIOL), 25 mM MgCl 2 (LightCycler 480 Genotyping Master, Roche) and PCR-grade water (LightCycler 480 Genotyping Master, Roche) to a total volume of 20 μl.

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Expressing

Journal: Regenerative Biomaterials

Article Title: An immunoregulatory and metabolism-improving injectable hydrogel for cardiac repair after myocardial infarction

doi: 10.1093/rb/rbae131

Figure Lengend Snippet: Characterization of the glucose metabolism regulating effect on cardiomyocytes of CFOD-BFZ hydrogel. ( A ) Schematic illustration of the mechanism for FBP release from CFOD-BFZ hydrogel to regulate the glucose metabolism and enhance ATP production. ( B–D ) Assays of the PK, PFK activity and the content of ATP in cardiomyocytes. ( E ) Cell viability assay of the cardiomyocytes treated with CSOD-BFZ hydrogel or CFOD-BFZ hydrogel under oxidative stress. ( F ) Assay of the activity of G6PDH activity in cardiomyocytes treated with CSOD-BFZ hydrogel or CFOD-BFZ hydrogel under oxidative stress. ( G ) Total antioxidant capacity of cardiomyocytes treated with CSOD-BFZ hydrogel or CFOD-BFZ hydrogel under oxidative stress. ( H ) Intracellular ROS level of cardiomyocytes treated with CSOD-BFZ hydrogel or CFOD-BFZ hydrogel under oxidative stress detected by DCFH-DA probe (* P < 0.05, ** P < 0.01, *** P < 0.001, n = 3).

Article Snippet: The ATP content assay kit, PFK activity assay kit, PK activity assay kit, G6PDH activity assay kit, 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) and total antioxidant capacity assay kit were purchased from Beyotime (Wuhan, China).

Techniques: Activity Assay, Viability Assay